The NR in ACR often removes hot pixels, so it might just be hiding it. However, I do not have that much noise even at ISO 3200 with my 5D MK II, so something is very strange with your camera, sensor, or DPP. My 5D MK III will be here next week, if I see problems, back it goes to stay, and I'll wait for the 1D X. For nearly twice the price of a 5D MK II, it should be very good.