mkamelg said:If you checked this lens (EF 100-400 mm f/4.5-5.6L IS II USM) on three different EOS 80D bodies and problem still existed there I think that there is no other explanation than problem with diffraction https://photographylife.com/what-is-diffraction-in-photography
http://www.the-digital-picture.com/Reviews/Camera-Specifications.aspx?CameraComp=792&Camera=1044
80D - extremly low value of pixel size 3.75µm, diffraction-limited aperture f/6.0
5D Mark III - pixel size 6.25µm, diffraction-limited aperture f/10.1
Someone drinked too much sake on some Canon's engineers integration party or what?
Take a shot with this combo (body plus lens) in RAW, run DPP (Digital Photo Professional) from Canon, download lens data profile and activate Digital Lens Optimizer. Is it better or not? If not I don't know what can help you.
This link below leads to fresh post from our unofficial Polish Canon related forum. Guy who posted it wrote himself that he has almost 10 years experience with aerial photography. He has also problem with sharp pictures from this combo from 250mm and up, especially on right side of the frame so maybe he has also problem with decentered lens but it's only my speculation. There's three example pictures inside.
http://www.canon-board.info/pierwsza-pomoc-awarie-usterki-problemy-56/prawa-strona-kadru-nieostra-rozjechana-zamydlona-canon-ef-100-400-ii-80d-106249/
Diffraction is a quality of the lens and not the sensor so I would say diffraction is not the issue.
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