zsolex said:
Hi,
I am new in macro photography.
When I got the EF 100mm /2.8 macro lens there are two ways to getting closer to the subject for bigger magnification.
- ie. 500D close-up filter
or
-extension tube
Please tell me what is the difference between twos in DOF or IQ and in another terms?
(Extension tube reduce light and need to have electrical contact to controll the aperture, close-up may reduce IQ)
So, you're looking to go over 1:1 magnification, right? I think you have the basics of the effect of the two ways to do that - an extension tube is just air, so no IQ effect, but the extra distance means loss of light and therefore longer exposures needed. A close-up lens will potentially decrease IQ (although the Canon 500D has only a minor impact because the optical quality is excellent). I assume you have the non-L version of the Macro (Canon doesn't make a 67mm close-up lens for the L macro, but the 58mm lenses will fit the non-L). When using an extension tube on a macro lens, DoF will be essentially the same as without the tube (i.e. very, very thin). Even though there is an apparent effect on aperture (loss of light), it's not a real effect, in that it doesn't alter DoF. A close-up lens will have no effect on DoF.
The other issue is how much additional magnification you can get. That works out to:
100mm f/2.8 Macro + 12mm extension tube - 1.17x
100mm f/2.8 Macro + 25mm extension tube - 1.38x
100mm f/2.8 Macro + 500D close-up lens - 1.21x
100mm f/2.8 Macro + 250D close-up lens - 1.41x
Generally, extension tubes are better for shorter focal lengths and close-up lenses are better for long focal lengths. 100mm is about the tipping point for that, so you can achieve similar maximum magnifications with either approach.
If you really want to go above 1:1, have you considered the MP-E 65mm? That lens starts where a normal macro lens stops, delivering 1x - 5x magnification.
Here's a shot with the MP-E 65mm at ~4x - this day lily stigma is about 5mm (0.2 inches) across:

EOS 5D Mark II, MP-E 65mm f/2.8 1-5x Macro @ 4x, 0.8 s, f/10, ISO 200