Can you use your 400mm f/2.8 for fast BIF? I once went on a Puffin shoot and got loads of great keepers of flying ones with the 400mm f/4 DO II but the guys with the 400/2.8 on tripods hardly got any because they weren't mobile enough.I agree that the market probably can't support two 400mm f2.8 lenses in Canon's line up. Thr original 400mm f2.8 mk1 was designed for sports and action. However, I use mine mostly in a wildlife context. I find it very versatile with or witout teleconverters. When ever looking at a S/H model, I usually find that the chrome lens mount takes a pounding with these lenses...far more than any other big white. So it's safe to assume that teleconverter use with these lenses is very common. You effectively get a 400/2.8, 600/f4 and a 800/f5.6 with the one lens.
In a wild life context, I find that I am backlighting on a fairly regular basis. So a DO lens (assuming the same issues with the mkII 400mm DO) for me isnt something that i would look for in a lens. I currently use a EF 400mm f2.8 LIS mk II and I can't see any reason to side grade currently.
I get the max frame rate that my R6ii is capable of. I get the best sharpness and IQ that I've ever got from a 400/2.8 and it's a lot lighter than the mk1. This lens is very portable compared to the mk1.
The RF version is just a mkIII EF version with an integrated EF to RF adapter and the difference between the EF mkIII and the EF mkII seems to be slight. I'm finding the mkII to be light and portable enough. Maybe that'll change for me when I'm another 10 eyars older!
A Do lens would be a lot smaller and lighter for sure, but will it be enough over the current RF version? It's possible that the DO prime would sit along side a more expensive zoom version. Say a DO option and a 100-400/f2.8 option.
Who know's except behind the closed doors of Canon lens design HQ.
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